- 表達(dá)克隆[通過(guò)進(jìn)行基因表達(dá)而克隆目的基因的方法
- 表達(dá)克隆化
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expression cloning中文什么意思
例句與用法
- In our study we have cloned the osd gene from s . typhimurium by pcr , characterized the gene product and used this gene to construct asd + expression cloning vectors ptrc99a - asd
再將hpylori尿素酶b亞單位基因與尿素酶b和熱休克蛋白a融合基因分別克隆入ptrc99a一asd質(zhì)粒的多克隆位點(diǎn)之內(nèi)。 - Conclusion constructed the high - level expression clone of echistatin in e . coli . the expression of recombinant protein is higher , it make the further study of echistatin feasible
結(jié)論成功構(gòu)建了echistatin的原核高效表達(dá)克隆，表達(dá)量高于現(xiàn)有國(guó)內(nèi)外研究水平，為echistatin功能及相關(guān)疾病的研究奠定了基礎(chǔ)。 - In the first part , the focus is to find the receptor molecules directly by screening two cdna libraries with a recombinant construct prpap or as an alternative , to find an enriched area in the embryo brains and construct libraries from this brain region and perform the expression cloning as above
方法： （ ）以融合蛋白prpap通過(guò)瞬時(shí)表達(dá)克隆法篩選兩個(gè)cdna文庫(kù)，或者通過(guò)與胚胎腦的結(jié)合實(shí)驗(yàn)篩選有豐富結(jié)合蛋白的腦區(qū)，以圖構(gòu)建cdna文庫(kù)并進(jìn)行表達(dá)克隆的篩選。 - Objective : construct high - level expression system of echistatin in e . coli methods : obtain amino - acid sequence of echistatin from genebank database . considering the bias of usage of 61 available aminoacid codons in e . coli , design the coding sequence of echistatin , synthesize the dna sequence chemically , get single copy coding gene and repeated two copy coding gene of echistatin . insert the sequence into expression vector pbv220 , and more , we construct fusion expression clone of echistatin with pcr , identify the recombinant vector by dna sequencing
目的構(gòu)建蛇毒鋸鱗蝰素( echistatin )的原核高效表達(dá)體系方法由genebank數(shù)據(jù)庫(kù)檢索蛇毒鋸鱗蝰素( echistatin )的氨基酸序列，結(jié)合大腸桿菌蛋白質(zhì)合成體系對(duì)氨基酸密碼子使用的偏愛(ài)性，設(shè)計(jì)了echistatin編碼基因，體外人工合成編碼基因dna片段，通過(guò)適當(dāng)?shù)南拗菩詢?nèi)切酶位點(diǎn)插入表達(dá)載體pbv220 ，分別構(gòu)建了echistatin的單拷貝表達(dá)克隆、雙拷貝串聯(lián)表達(dá)克??；進(jìn)一步通過(guò)pcr技術(shù)構(gòu)建echistatin的融合表達(dá)基因克隆。
百科釋義
Expression cloning is a technique in DNA cloning that uses expression vectors to generate a library of clones, with each clone expressing one protein. This expression library is then screened for the property of interest and clones of interest recovered for further analysis.
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